High-performance liquid chromatography (HPLC) elution data provide a useful tool for quantitative glycosylation profiling, discriminating isomeric oligosaccharides. The web application GALAXY (Glycoanalysis by the Three Axes of MS and Chromatography), which is based on the three-dimensional HPLC map of N-linked oligosaccharides with pyridyl-2-amination developed by Dr. Noriko Takahashi, has been extensively used for N-glycosylation profiling at molecular, cellular, and tissue levels. Recently we updated GALAXY as version 3, which includes new HPLC data including those of glucuronylated and sulfated glycans, an improved graphical user interface using modern technologies, and linked to glycan information in GlyTouCan and the GlyCosmos Portal. This liaison will facilitate glycomic analyses of human and other organisms in conjunction with multi-omics data.
We would like to express our profound respect and gratitude to the late Dr. Noriko Takahashi, Professor Emeritus of Nagoya City University, who developed HPLC mapping method and collected most of the HPLC data registered in the GALAXY system. This work was supported in part by the National Bioscience Database Center and Japan Science and Technology Agency.
Yagi, H., Amagasa, E., Shiota, M., Yamada, I., Aoki-Kinoshita, K.F. and Kato, K. GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map Glycobiology 32, 646-650 (2022)
https://doi.org/10.1093/glycob/cwac025
All copyrightable parts of the datasets in GALAXY ver3 are under the Creative Commons Attribution (CC BY 4.0) License. Please refer to Policies for details.
Contact: glycosmosup@gmail.com
Last updated: 21 Dec, 2022.